BRCA1, BRCA2 mutations

Authors Samah Lutfi, Nasra F Abdel-Fattah, Ahmed Barakat, Omar Al-Farouq, Tariq Hashem, Ahmed Muhammad Othman, Shaima Metwally, Maha Abu Shadi, Amani Hilal, Shaza Habib, Ahmed Al-Sharif, Abdel-Wahhab Al-Gharib, Muhammad Munir, Manar Munir, Marwa Abdel-Wahed Mona Salem, Asmaa M. Salama, Fatima Hafez, Sarah Ajwa, Hisham El-Ghazaly, Manal El-Mahdy, Mark J Dunning, Sherif F. ThursdayPosting date 7/1/2021 Cancer Research Source sound 81 Case 13 Appendix pp. 2512-2512 Publisher American Association for Cancer Researcha description INTRODUCTION: Breast cancer (BC) is a multifactorial disease, attributable to non-familial factors such as environmental or genetic factors that play a vital role in the development of the disease. BRCA1/2 mutations represent a significant risk of breast cancer. The current study focused on exploring the relationship between the presence of BRCA1/2 mutations and some clinicopathological characteristics that may affect the pathogenesis of BC. MATERIALS AND METHODS: Genomic DNA samples were obtained from 19 fresh tissues using a Gene Jet extraction kit (Thermo Scientific/USA, Canada), and 29 FFPE samples using a QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, USA) of BC patients. Library preparation was performed using the Devyser BRCA NGS kit (DEVYSER, Stockholm, Sweden), according to the manufacturer’s instructions for 9 s. Reagent kit V2, 500 Cycles PE, on an Illumina MiSeq